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Objective The purpose of this study is to grasp the current situation of radiation protection in some non-medical institutions in Hangzhou, and to provide basis and reference for the relevant authorities departments to make the radioactive hazard factors monitoring plan for non-medical institutions. Methods The configuration of the personnel protective equipment and radiation level of radiation sources and radiation devices in 5 non-medical monitoring institutions in Hangzhou were investigated and analyzed by means of questionnaire survey and on-site detection. Results The radiation workers of 5 monitoring institutions have carried out personal dose monitoring, and their annual individual dose equivalent meets the requirements of relevant national standards. The ambient dose equivalent rates around the radiation source and radiation device room are lower than the minimum detection limit of the instrument. When the source is stored, the maximum ambient dose equivalent rates at 5 cm and 100 cm away from the external surface of the source container were 22.2 μSv/h and 2.0 μSv/h, respectively. When the source is in use, the maximum ambient dose equivalent rates at 5 cm and 100 cm away from the external surface of the source container were 44.3 μSv/h and 5.0 μSv/h, respectively. Conclusion Under normal operation, the radiation dose level of some non-medical monitoring institutions in Hangzhou is at a low level, while the ambient dose equivalent rates around the external surface of the source container is at a high level. Therefore, non-medical institutions should improve their awareness of radiation protection and strengthen radiation management.
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BACKGROUND@#Both aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphism and lifestyle behaviors are involved in coronary artery disease (CAD), while the interaction between them is currently unknown.@*METHODS@#A nested case-control study was conducted in 161 patients with CAD and 495 controls in dyslipidemia population in Yinzhou District, Ningbo, Zhejiang Province, China, in August 2013. Anthropometric data and blood samples were collected, demographic characteristics and lifestyle behaviors information were obtained by a face-to-face interview, dietary intake was assessed by a food frequency questionnaire, and genomic DNA was genotyped.@*RESULTS@#Carriers with increasing number of A alleles had an elevated CAD risk compared with G allele carriers (adjusted OR = 1.483, 95% CI = 1.114-1.974). Carriers of rs671 A/G and A/A genotypes had a higher CAD risk than carriers of G/G genotype (adjusted OR = 1.492, 95% CI = 1.036-2.148). Similarly, individuals with rs671 A/A genotype had a higher CAD risk than individuals with A/G and G/G genotypes (adjusted OR = 2.161, 95% CI = 1.139-4.101). We found a borderline additive interaction between regular fried food intake and A/A and A/G genotypes, and a significantly additive interaction between sedentary/light physical activity and A/A and A/G genotypes.@*CONCLUSIONS@#Individuals with A/A or A/G genotypes of rs671 have a higher CAD risk, if they lack physical activity and take fried food regularly, than individuals with G/G genotypes. These findings can help to provide a guide to targeted heart health management.
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Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Aldehyde Dehydrogenase, Mitochondrial , Genetics , Alleles , Case-Control Studies , China , Coronary Artery Disease , Blood , Genetics , Dyslipidemias , Blood , Genetics , Genetic Association Studies , Genetic Predisposition to Disease , Genotype , Life Style , Polymorphism, Single Nucleotide , Risk FactorsABSTRACT
Objective@#To establish a method for determination the S-phenylmercapturic acid in urine by dispersive solid-phase extraction using Humic Acid/Fe3O4 magnetic nanocomposite as adsorbent.@*Methods@#The 5 ml of urine samples were adjusted to pH 1.0 and extracted by Fe3O4@HA. Then the analytes were separated on EC-C18 capillary column and detected by HPLC-VWD. The S-phenylmercapturic acid was characterized by the retention time and quantified by peak area and external standard method.@*Results@#The standard curves of SPMA showed significant linearity between 0.04~1.00 mg/L (r=0.999 7) . The average recovery was 94.2%~102.4%. The intra-day and inter-day precisions (RSD) were 2.9~6.7% (n=6) and 3.1~7.5% (n=6) respectively. The detect limit of SPMA was 0.012 g/L (S/N=3) .@*Conclusion@#This method is simple, rapid, sensitive and accurate. It is applicable for determination of SPMA in the urine of works who were exposed to benzene.
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Social evaluation governance strategy was introduced to study the social management path for public hospitals.On the basis of definition of social governance and social evaluation theory, clarification of the concepts and relationship logic of public hospital′s social evaluation,and analysis of the context analysis of their social evaluation policy,the paper discussed the establishment of social evaluation and governance theory logic framework of public hospitals.In terms of such realization paths as the purposes,indexes,entities,and methods of the evaluation,as well as result release and application of the evaluation,recommendations were made.
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Objective To survey the cognitive attitude and participation intention for stakeholders of public hospitals,in order to provide basis for public hospitals social evaluation.Methods 621 stakeholders of public hospitals were subject to the questionnaire survey.Results 76.7%(475/619) stakeholders held it as necessary to independently launch the public hospitals social evaluation,and held that the evaluation subjects selection should be professional(83.8%),independent(67.7%),authoritative (55.6%),and proactive(46.1%).The survey also recommended that social evaluation of public hospitals should have such indexes as government investment (80.2%),social supervision (76.0%),and information disclosure system(7 1.1%).The overall willingness rate of stakeholders to participate in the public hospital social evaluation was 7 1.6%.Conclusions Development of the social evaluation index system of public hospitals and diversified evaluation subj ects are key to developing the theory and practice system of public hospitals social evaluation.
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Aim To explore the effect of miRNA-143 ( miR-1 4 3 ) on homocysteine ( Hcy ) induced-vascular smooth muscle cells ( VSMCs ) proliferation and the mechanism .Methods VSMCs were cultured and in-cubated with Hcy by using primary cultured method . Then, cells were treated with different concentrations of Hcy and folate .VSMCs proliferation was determined with MTT assay , miR-143 was measured by qRT-PCR, and methylation of miR-143 was determined with meth-ylated PCR.Results After cells were treated with dif-ferent concentrations of Hcy , the proliferation of VSMCs was significantly increased , mRNA expression of miR-143 was decreased and methylation of miR-143 was increased .The proliferation of VSMCs was signifi-cantly decreased when transfected VSMCs with miR-143 precursor , and cell proliferation was increased by using miR-143 inhibitor transfection .Conclusion Hy-pomethylation of miR-143 may inhibit VSMCs prolifera-tion.
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[Summary] The study was to introduce quality of life measurement instruments for thyroid cancer,as well as the application of these scales.(1) Quality of life scales such as generic scale SF-36,tumor-specific scale EORTC QLQ-C30 were used in previous studies.(2) In these studies,the quality of life assessment was mainly used for measurement of quality of life in patients with thyroid cancer,comparative effectiveness research and specific psychological intervention evaluation,analysis of relevant factors affecting quality of life in patients with thyroid cancer.
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Aim To investigate the role of miR-125 b and its DNA methylation in homocysteine ( Hcy )-in-duced vascular smooth muscle cells( VSMCs) prolifera-tion. Methods VSMCs were stimulated with 0,50, 100, 200, 500 μmol · L-1 Hcy respectively. Then qRT-PCR was used to detect the mRNA levels of miR-125b,and nested-touchdown methylation-specific PCR ( ntMS-PCR) was used to detect the methylation levels of miR-125b. VSMCs were transfected with miR-125b precursor or the inhibitor of miR-125b ,then 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide ( MTT ) assay was used to reflect the proliferation of VSMCs. The distribution of CpG islands of miR-125b promoter region was analyzed by bioinformatics meth-ods. VSMCs were stimulated with 100 μmol·L-1 Hcy and transfected with or without DNA methylation inhib-itors 5-nitrogen impurity cytidine ( AZC) , then the ex-pression of miR-125b was detected by qRT-PCR. Re-sults The mRNA levels of miR-125 b were decreased in 100,200,500 μmol·L-1 Hcy group compared with 0 μmol·L-1 Hcy group. The precursor of miR-125b could inhibit the proliferation activity and the inhibitor of miR-125 b could increase the proliferation activity of VSMCs cells. Bioinformatics analysis indicated that MiR-125 b promoter region had a CpG island whose length was 792 bp ( 1881-2672 ) . The miR-125 b pro-moter region methylation levels increased after Hcy in-tervention ( P <0. 01 ) . The expression level of miR-125 b increased after AZC intervention ( P <0. 05 ) . Conclusions ① Hcy promotes vascular smooth mus-cle cell proliferation maybe by down-regulating the ex-pression of miR-125b. ② Hcy down-regulates the ex-pression of miR-125 maybe by up-regulating the methy-lation levels of miR-125b promoter region.
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Objective To explore the association between walking time and the perception of built environment among local adults in Hangzhou.Methods Through multistage stratified random sampling,a total of 1 440 urban residents aged 25-59 years were surveyed in Hangzhou by face-to face interview in 2012.The international physical activity questionnaire-long version (IPAQ-L) was used to assess the physical activity levels,including walking time in the past week.Neighborhood Environment Walkability Scale-Abbreviated (NEWS-A) was used to obtain information about their perception of built environment.Multiple logistic regression was applied to estimate the relationship between waking and the perception of built environment.Results Among the local adults in Hangzhou,the median of total physical activity was 2 766 met · min-1 · week 1,the average walking time per week was 90 min for leisure and 100 min for transportation respectively.After controlling the age,marital status,BMI,educational level,employment,community type and the total PA scores,the leisure-time walking was negatively related to the accessibility to stores,facilities and other things for both man (OR=0.764,95％CI:0.588-0.992) and woman (OR=0.633,95％CI:0.481-0.833).In sex specific analysis,the leisure-time walking was negatively related with the residential density (OR=0.997,95％CI:0.996-0.999) while transportation related walking was positively related with walking/cycling way scores (OR=1.537,95％ CI:1.138-2.075) in females.In contrast,there were no significant associations between perception of built environment and transportation related walking in males.Conclusion Improving the built environment,such as the walking/cycling way,might be useful to increase the transportation related walking time for adults.The sex specific differences need to be considered in the environment intervention for walking promotion.
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Objective To understand the perceptions of physical activity-related built environment among urban adults in Hangzhou.Methods A face-to-face interview was conducted among the urban residents aged 25-59 years selected through multistage stratified random sampling in Hangzhou in 2012.The Neighborhood Environment Walkability Scale-Abbreviated (NEWS-A) was used to assess the perception of built environment among residents,including residential building density,the diversities of stores,facilities and others,the accessibility to public service,the street connectivity,walking/cycling facilities,aesthetics,traffic safety,and public security.The multilevel logistic regression model was used to assess the demographic characteristics,BMI and other factors' influence on people' s perceptions.Results Among 1 362 local residents surveyed,no sex,martial status and occupation specific significant differences in the perception of built environment were found.After adjusting other factors,the age group 45-59 years was positively related to the score of street connectivity (OR=2.02,95％ CI:1.30-3.15).The educational level of college or higher was positively associated with the score of residential building density(OR=1.97,95％CI:1.29-3.00) but negatively associated with the score of facility variety(OR=0.65,95％ CI:0.43-0.97).Overweight or besity was negatively related to the scores of walking/cycling ways (OR=0.67,95％CI:0.48-0.95) and public security(OR=0.75,95％ CI:0.57-0.99).Compared with the class Ⅰ residential area,the people in class Ⅲ residential area had lower perception scores on facility diversity (OR =0.11,95％ CI:0.04-0.30),accessibility to public service (OR=0.33,95％ CI:0.11-0.95),street connectivity (OR=0.30,95％ CI:0.11-0.86) and traffic safety (OR=0.39,95％ CI:0.17-0.91).Conclusion The perceptions of physical activity-related built environment was associated with age,educational level,BMI and residential area.The personal characteristics should be considered while performing environment intervention on physical activity.
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Objective To evaluate the effect of the Oxford Health Alliance-Community Interventions for Health on chronic diseases prevention and control capacity in community medical staff in Hangzhou.Methods A two year comprehensive intervention on chronic disease prevention and control capacity was conducted in the medical staff in Xiacheng district and Gongshu district,Xihu district was used as control according to the study design principal of parallel comparison and non-random grouping.The intervention effect was evaluated with Cochran-Mantel-Haenszel test.A total of 985 questionnaires were completed in the baseline survey and 870 questionnaires were completed in the follow-up survey.Results After 2 year intervention, the detection rates of blood cholesterol, blood pressure and fasting blood glucose by community medical staff significantly increased compared with the rates before the intervention (x2 =7.97, P =0.05;x2 =27.21, P =0.00;x2=21.32, P=0.00).While, in the control district, the fasting blood glucose detection rate increased from 25.37％ to 31.19％, the differences showed statistical significance (x2=15.29, P=0.00).The communication between community medical staff and patients about healthy diet pattern was improved after the intervention, the difference was statistical significant (x2=8.64, P=0.03).However, no significant differences in communication about increasing physical activity and smoking cessation between community medical staff and patients was found before and after intervention both in intervention districts and in control district.Furthermore, multivariate logistic regression analysis indicated that the interventions on screening of common chronic diseases and management of chronic diseases patients were related with the detections of blood cholesterol, blood pressure and fasting blood glucose.Moreover, the intervention on counsel and suggestion for chronic disease prevention and control had some influence on the communication about chronic disease risk factors between community medical staff and patients.Conclusion The intervention on chronic disease prevention and control capacity in community medical staff was effective to improve the detection of chronic diseases in community and the communication between the medical staff and patients.
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<p><b>OBJECTIVE</b>To explore the level of physical activities (PA) and related influencing factors among junior high school personnel in Hangzhou.</p><p><b>METHODS</b>In a cross sectional study, a self administered questionnaire was used to assess the levels of PA. Kruskal-Wallis H test and ordinal regression were applied to analyze the related factors.</p><p><b>RESULTS</b>Among the 1 497 school personnel included in this study, prevalence rates on the low, moderate and high levels of PA were 40.8%, 40.6% and 18.6%, respectively. Data from the multinomial logistic regression analysis indicated that:when comparing with the low level, people aged ≥ 45 (OR = 1.576, 95% CI:1.128-2.201) or those having minor perceived stress (OR = 1.490, 95% CI:1.141-1.946) more likely to fall into the moderate level. However, factors as higher awareness scores on the benefit of PA (OR = 1.070, 95%CI:1.002-1.143), being male (OR = 3.088, 95%CI:2.162-4.409), always or very often taking PA together with colleagues (OR = 2.903, 95%CI:1.587-5.312), taking PA once in a while together with colleagues (OR = 1.900, 95%CI:1.160-3.110) or having space to walk around home (OR = 1.657, 95% CI:1.022-2.688) would increase the likelihood of having high PA level. However, factors as marital status, socioeconomic status, years of working at school, BMI, level of having received education and positive impact from colleagues etc. did not seem to have significantly related to the PA level.</p><p><b>CONCLUSION</b>Gender, age, stress from work and supportive environment should be taken into account while planning on promoting the PA level among junior high school personnel in Hangzhou.</p>
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Adult , Female , Humans , Male , Middle Aged , China , Cross-Sectional Studies , Exercise , Faculty , Logistic Models , Surveys and QuestionnairesABSTRACT
Objective To investigate the hand hygiene status of urban and rural residents in Hangzhou City of Zhejiang Province to provide scientific base for health promotion.Methods Urban residents,rural residents,personnel in government institutions,urban students,rural students and enterprise workers were enrolled in this investigation.By using constant volume sampling method,fifty individuals in each group were recruited from 13 districts of Hangzhou City,and a total of 3 337 people filled in the questionnaire,which included demographic characteristics,knowledge of hand hygiene and hand washing.Logistic regression analysis and x2 test were used for data analysis.Results Correct hand-washing was found in 35.84％ urban and rural residents (urban vs.rural:41.45％ vs.27.06％).Awareness rate of hand hygiene was 35.06％.The influencing factors of proper hand washing were location,gender,age,educational level and understanding of hand hygiene.The influencing factors of awareness of hand hygiene were location,gender,age and educational level.Conclusion Hangzhou citizens had a relatively lower rate of proper hand washing,and significant difference was found between urban and rural residents; the study suggested that para-urben residents need to improve their knowledge and behavior of hand hygiene.Meanwhile,the students urgenty need to improve their hand hygiene
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Aim To explore the role of ERO1 αand its DNA methylation in homocysteine (Hcy)-induced in-hibition of hepatocytes proliferation.Methods The hepatocytes stimulated with 0 μmol·L -1 Hcy were set as the normal group (NC group)and the hepatocytes stimulated with 1 00 μmol·L -1 Hcy as the experimen-tal group (Hcy group).Methyl thiazolyl tetrazolium (MTT)reduction assay was used to reflect the prolifer-ation of the hepatocytes;qRT-PCR and Western blot were used to detect the mRNA and protein levels of ERO1 α;the expression of green fluorescence protein was observed in hepatocytes after the recombinant plas-mid of ERO1 α was constructed,which was used to confirm if the recombinant plasmid into hepatocytes was successful,then the mRNA and protein levels of ERO1 αwere assayed and the proliferation of the hepa-tocytes was also detected;ntMSP was used to detect the change of ERO1 αDNA methylation.Results The mRNA and protein levels of ERO1 αwere decreased in Hcy group compared with NC group,and the prolifera-tion activity of hepatocytes in Hcy group was de-creased.Sequencing result showed that the recombi-nant plasmid of ERO1 αwas constructed successfully. QRT-PCR and Western blot revealed that ERO1 αwas overexpressed. The result of MTT suggested that ERO1 αoverexpression restored hepatocyte proliferation inhibited by Hcy.Hcy caused ERO1 αDNA hyperm-ethylation.Conclusions Hcy inhibits hepatocyte pro-liferation by downregulating the expression of ERO1 α, and methylation of ERO1 αpromoter may play a role in this process.
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Objective To explore the level of physical activities (PA) and related influencing factors among junior high school personnel in Hangzhou. Methods In a cross sectional study,a self administered questionnaire was used to assess the levels of PA. Kruskal-Wallis H test and ordinal regression were applied to analyze the related factors. Results Among the 1 497 school personnel included in this study,prevalence rates on the low,moderate and high levels of PA were 40.8%,40.6%and 18.6%,respectively. Data from the multinomial logistic regression analysis indicated that:when comparing with the low level,people aged ≥45(OR=1.576,95%CI:1.128-2.201) or those having minor perceived stress(OR=1.490,95%CI:1.141-1.946)more likely to fall into the moderate level. However,factors as higher awareness scores on the benefit of PA(OR=1.070, 95%CI:1.002-1.143),being male(OR=3.088,95%CI:2.162-4.409),always or very often taking PA together with colleagues(OR=2.903,95%CI:1.587-5.312),taking PA once in a while together with colleagues(OR=1.900,95%CI:1.160-3.110)or having space to walk around home(OR=1.657, 95%CI:1.022-2.688) would increase the likelihood of having high PA level. However,factors as marital status,socioeconomic status,years of working at school,BMI,level of having received education and positive impact from colleagues etc. did not seem to have significantly related to the PA level. Conclusion Gender,age,stress from work and supportive environment should be taken into account while planning on promoting the PA level among junior high school personnel in Hangzhou.
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Objective To explore the level of physical activities (PA) and related influencing factors among junior high school personnel in Hangzhou. Methods In a cross sectional study,a self administered questionnaire was used to assess the levels of PA. Kruskal-Wallis H test and ordinal regression were applied to analyze the related factors. Results Among the 1 497 school personnel included in this study,prevalence rates on the low,moderate and high levels of PA were 40.8%,40.6%and 18.6%,respectively. Data from the multinomial logistic regression analysis indicated that:when comparing with the low level,people aged ≥45(OR=1.576,95%CI:1.128-2.201) or those having minor perceived stress(OR=1.490,95%CI:1.141-1.946)more likely to fall into the moderate level. However,factors as higher awareness scores on the benefit of PA(OR=1.070, 95%CI:1.002-1.143),being male(OR=3.088,95%CI:2.162-4.409),always or very often taking PA together with colleagues(OR=2.903,95%CI:1.587-5.312),taking PA once in a while together with colleagues(OR=1.900,95%CI:1.160-3.110)or having space to walk around home(OR=1.657, 95%CI:1.022-2.688) would increase the likelihood of having high PA level. However,factors as marital status,socioeconomic status,years of working at school,BMI,level of having received education and positive impact from colleagues etc. did not seem to have significantly related to the PA level. Conclusion Gender,age,stress from work and supportive environment should be taken into account while planning on promoting the PA level among junior high school personnel in Hangzhou.